Effect of azelastine hydrochloride nasal spray combined with desloratadine to inflammatory factors, cell function and IgE of patients with allergic rhinitis / 中国生化药物杂志

Objective To explore the effect of azelastine hydrochloride nasal spray combined with desloratadine to inflammatory factors, cell function and IgE of patients with allergic rhinitis.Methods 92 cases of allergic rhinitis patients treated in the first affiliated hospital of wenzhou medical college hospital from June 2014 to December 2015 were divided into experimental group(n=46) and control group(n=46) according to the random number table method.The control group was given oral loratadine tablets, one piece per time, one time per day, while the experimental group was given azelastine hydrochloride nasal spray on the basis of the control group,each nostril one spray, one time in the morning and night.The clinical efficacy of two groups of patients would be observed after 4 weeks,ELISA would be used to detect serum levels of IFN-γ, IL-4, IL-8 and IgE level, and IFN-γ/IL-4 was the value of Thl/Th2,flow cytometry instrument was used to the determination of T cell subgroup CD4 +,CD8 + cells.Results 4 weeks after treatment,stuffy nose, nasal itching, runny nose, sneezing and nasal cavity change points are lower than before the treatment in both the two groups.Experimental group obviously lower than the control group, the difference was statistically significant ( P <0.05 ).The total effective rate of treatment group is higher than the control group,the difference was statistically significant(χ2 =4.389,P=0.036).The serum level of IFN-γis higher than before treatment in both the two groups.IL-4, IL-8 inflammatory factor levels were lower than before treatment,the experimental group was better than control group,the difference was statistically significant(P<0.05).CD4 +,CD8 +of T cells and Thl/Th2 values are higher than before the treatment in both the two groups,the experimental group was higher than control group, the difference was statistically significant ( P<0.05 ).Serum IgE levels were lower than before the treatment in both the two groups,the experimental group was lower than control group,the difference was statistically significant (P<0.05).Conclusion The therapy of azelastine hydrochloride nasal spray combined with desloratadine can improve the clinical effect of the treatment of allergic rhinitis,reduce inflammation,strengthen the body's immune function, improve thelevel of serum IgE.

Activation of mTOR signaling pathway in cancer stem cells of nasopharyngeal carcinoma and inhibitive effect of rapamycin against the cancer stem cells / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the mTOR expression of cancer stem cells(CSCs) in nasopharyngeal carcinoma and preliminarily explore the mechanism of inhibiting its proliferation with rapamycin.@*METHOD@#Nasopharyngeal carcinoma spherical cells were gathered by using serum-free suspension culture method, CCK8 assay was used to detect cell proliferation, Western blot assay was used to detect the expression of CD44, OCT4, SOX2 and mTOR signaling. The spherical cells and CNE2 were treated with rapamycin in concentrations of 0, 0.1, 1.0, 10.0, 100.0, 1000.0 nmol/L, CCK8 assay was used to detect cell inhibition ratio, Western blot assay was used to detect the expression of mTOR signaling of nasopharyngeal carcinoma spherical cells.@*RESULT@#Compared with CNE2, the spherical cells exhibited a high proliferation rate in RPMI 1640 medium supplemented with fetal bovine serum, and overexpressed in OCT4, SOX2 (P 0.05). Although the expression levels of mTOR, P70S6, 4EBP1 were not significantly different between the two kinds of cells (P > 0.05) the proteins of phosphorylation activation form of them (P-mTOR, P-P70S6, P-4EBP1) were highly expressed in spherical cells (P < 0.05). The spherical cells and CNE2 were treated with rapamycin in different concentrations, the concentrations for 50% of maximal effect of spherical cells and CNE2 were 2.59 nmol/L and 78.12 nmol/L respectively, rapamycin inhibited the spherical cells more strongly compared with CNEZ. The expression levels of P-mTOR, P-70S6, P-4EBP1 in spherical cells were gradually decreased with increasing of the concentrations of rapamycin, but the difference of the expression levels of mTOR, P70S6, 4EBP1 were not significant.@*CONCLUSION@#The proteins of mTOR signaling pathway of CSCs in nasopharyngeal carcinoma are overexpressed, and rapamycin can effectively inhibit cell proliferation of CSCs in nasopharyngeal carcinoma by blocking mTOR signaling pathway.

Investigation of KGF and KGFR expression in external auditory canal skin adjacent to tympanic membrane perforation / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the continuous expression of keratinocyte growth factor(KGF)and keratinocyte growth factor receptor(KGFR)in external auditory canal skin adjacent to tympanic membrane perforation and analyze the role of KGF and KGFR in the different turnover of chronic otitis media.METHODS The external auditory canal skin adjacent to tympanic membrane perforation from 20 cases with cholesteatoma otitis media and the corresponding cholesteatoma tissue and normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis.The positive rate was compared with 20 cases of non-cholesteatom otitis media's external auditory canal skin adjacent to tympanic membrane perforation.RESULTS The staining for KGF and KGFR in cholesteatoma otitis media's external auditory canal skin adjacent to tympanic membrane perforation was consistently stronger than that in non-cholesteatoma otitis media.The positive rates of the two tissue was(33.135?6.364)% and(19.965?10.570)%,(19.380?2.827)% and(13.145?7.935)% respectively,revealing a significant difference.CONCLUSION The activity of hyperproliferation of the external auditory canal skin adjacent to tympanic membrane perforation in cholesteatoma otitis media is stronger than that of non-cholesteatoma otitis media.KGF and KGFR may play a more important role for hyperproliferation of cholesteatoma.

The expression and significance of keratinocyte growth factor in the regulation of cholesteatoma growth / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the expression of keratinocyte growth factor(KGF)in middle ear cholesteatoma and to explore the role of KGF on the hyperproliferation of the cholesteatoma epithelium and the formation and development of cholesteatoma. METHODS The specimens from the cholesteatoma tissue of 20 cases and the corresponding normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis. RESULTS In normal ear skin only stroma staining for KGF was positive. In cholesteatoma epithelium staining for KGF was strongly positive and the stroma staining was stronger than that of normal ear skin. The positive rates of the cholesteatoma and normal external ear skin revealed a significant difference. There was a positive correlation between cholesteatoma epithelium staining for KGF and for Ki67 and the coefflcient of correlation was 0.609(P﹤0.01). CONCLUSION There was correlation between the expression of KGF or Ki67 and the ability of reproduction of middle ear cholesteatoma. Local inflammation might promote hyperproliferation of the epithelium of cholesteatoma by regulating the expression of KGF. It suggested that an autocrine stimulation of KGF correlate with the occurrence and development of cholesteatoma .